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Ph.D., 1989, Mechanical Engineering, Massachusetts Institute of Technology

M.S., 1985, Mechanical Engineering, Massachusetts Institute of Technology

B.S., 1983, Mechanical Engineering, Iowa State University

Research

Functional Corneal Stromal Engineering:

This project focuses on the development of a functional corneal equivalent with optical and biomechanical properties comparable to those found in the native cornea. One objective involves understanding the role of initial matrix processing and composition on the properties of the corneal equivalent. Unlike other materials, the properties of tissue are influenced by cell/matrix interactions. The deposition, contraction and degradation of the matrix by the cells are significant influences on the properties of the corneal equivalent. We are interested in characterizing and controlling those interactions. Finally, the macroscopic properties of the corneal equivalent reflect the microstructure of the matrix. We are also interested in characterizing matrix microstructure with time in culture and correlating microstructure with macroscopic properties via theoretical models.

Hematopoietic Cell Processing and Preservation:

Blood and blood products comprise approximately 2 % of the healthcare dollars in the United States. As therapies based on hematopoietic cells continue to become the standard of care for a range of medical conditions, effective methods of processing and preserving hematopoietic cells used therapeutically are important. We are interested in the development of: (1) short term storage solutions; (2) optimized methods of cryopreservation; (3) post thaw processing of the cells prior to use. These protocols must be integrated into closed-systems for cell processing and may also involve the design of supportive equipment for these processes.

Nanoparticle Facilitated Transport:

Nanoparticle/cell interactions are important for a variety of applications including drug delivery and health affects of nanoparticles. We are interested in characterizing the kinetics of nanoparticle/cell interactions via caveolar uptake, an active intracellular transport mechanism. We have used surface modified quantum dots to visualize attachment and internalization of nanoparticles. Our studies also suggest that transport of these nanoparticles in tissue differs greatly from passive diffusion. We have developed methods using near infrared imaging to monitor particle transport in tissue or animal systems. These studies will be important in controlling/modulating nanoparticle cell and tissue interactions.

Preservation of engineered tissues:

The biological and biophysical properties of engineered tissues vary from that of a native tissue. We are interested in the determination of thermophysical, biophysical and biomechanical properties of engineered tissues. This information can be integrated into theoretical modeling and validated experimentally. The resulting protocols will be designed to optimize post thaw viability and physical integrity/strength.