University of Minnesota
University of Minnesota: Department of Mechanical Engineering
http://www.me.umn.edu/

Allison Hubel Publications

Book Chapters

Hubel, A. Cryopreservation of cells used therapeutically, In: Cellular Therapy: Principles, Methods and Regulations, AABB Press, Bethesda, MD, 2008.

Hubel A. Cellular Preservation - Gene Therapy, Cellular Metabolic Engineering. In: J.G. Baust (ed). Advances in Biopreservation, Boca Raton, FL, CRC Press, 2006.

Journal Articles

Rudisill S, Divito MD, Stein A, Hubel A, “In vitro Collagen Fibril Alignment via Incorporation of Nanocrystalline Cellulose, Acta Biomaterialia, doi:10.1016/j.actbio.2014.10.024.

Hubel A, Spindler R, Curtsinger J, Lindgren B, Wiederoder S, McKenna DH, “Postthaw characterization of umbilical cord blood: markers of storage lesion”, Transfusion, DOI: 10.1111/trf.12971.

Hubel A, Spindler R, Skubitz A, “Storage of human biospecimens: selection of the optimal storage temperature”, Biopres & Biobanking, 12(3): 165-175, 2014.

Sharma RR, Pollock K, Hubel A, McKenna D, “Mesenchymal stem or stromal cells: a review of clinical applications and manufacturing practices”, Transfusion, 54(5): 1418-1437, 2014.

Pollock K, Sumstad D, Kadidlo D, McKenna DH, “Clinical mesenchymal stromal cell products undergo functional changes in response to freezing”, Cytotherapy, 17(1): 38-45, 2014.

Lam CH, Hansen EA, Hall W, Hubel A. “Application of transport phenomena analysis technique to cerebrospinal fluid”, J Neurosurg Sci, 57: 317-326, 2013.

Pollock K, Albares A, Wendt C, Hubel A. “Isolation of fibroblasts and epithelial cells in Bronchoalveolar Lavage (BAL)”, Exp. Lung Res, 39: 146-154, 2013.

Sharma A, Zhu Y, Thor SS, Hubel A, Stadler B. “Magnetic barcode nanowires for osteosarcoma cell control, IEEE Trans. Magnetics, 49: 453-456, 2013.

Lam CH, Hansen EA, Jansson C, Bryan A, Hubel A, “The characterization of arachnoid cell transport II: paracellular transport and blood-cerebrospinal fluid barrier formation, Neuroscience, 222: 228-238, 2012.

Hanna J, Hubel A, Lemke E, “Diffusion-based extraction of DMSO from a cell suspension in a three stream, vertical microchannel, Biotech. & Bioengr, 109: 2316-2324, 2012.

Bala Chandaran R, Reinhart J, Lemke E, Hubel A, “Influence of buoyancy-driven flow on mass transfer in a two-stream microfluidic channel: Introduction of cryoprotective agents into cell suspensions”, http://dx.doi.org/10.1063/ 1.4767463, 2012.

DiVito MD, Rudisill SG, Stein A, Patel SV, McLaren JW, Hubel A, “Silica hybrid for corneal replacement: optical, biomechanical and ex vivo biocompatibility studies, doi:10.1167/iovs.12-10561, 2012.

Unhale SA, Solomon R, Skubitz APN, Hubel A, “Stabilization of tissue biospecimens for pathological examination and biomedical research, Biopres & Biobanking, 10 (6): 2012.

Hubel A. “Advancing the preservation of cellular therapy products,” Transfusion, (S1, Supplement 4): 82S-86S, 2011.

Lam Ch, Hansen EA, Hubel A, “Arachnoid cells on culture plates and collagen scaffolds: phenotype and transport properties”, Tissue Engineering, 17:1759-1766, 2011

Hubel A, Aksan A, Skubitz APN, Wendt C, Zhong X, “State of the art in preservation of fluid biospecimens”, Biopreservation & Biobanking,9:237-244, 2011

Ragoonanan V, Hubel A, Aksan A, “Response of the cell membrane-cytoskeleton complex to osmotic and freeze/thaw stresses”, Cryobiology, 61(3): 335-344, 2010.

Dong JP, Malsam J, Bischof JC, Hubel A, Aksan A, “Spatial distribution of the state of water in frozen mammalian cells”, Biophys. J, 99: 2453-2459, 2010.

Janson C, Romanova L, Hansen E, Hubel A, Lam C, “Immortalization and functional characterization of rat arachnoid cell lines”, Neuroscience, doi:10.1016/j.neuroscience.2010.12.035.

McCullough J, Haley R, Clay M, Hubel A, Lindgren B, Morof G, “Long-term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer”, Transfusion, 50(4): 808-819, 2010.

Mata C, Longmire EK, McKenna DH, Glass KK, Hubel A. “Cell motion and recovery in a two-stream microfluidic device,” Microfluid & Nanofluid, 8: 457-465, 2010

Aksan, A, Hubel, A, Bischof, JC, Frontiers in biotransport: water transport and hydration, J. Biomech Engr, 131(7), 074004, 2009.

Dong, J, Hubel, A, Bischof JC and Aksan, A, Freezing-induced phase separation and spatial microheterogeneity in protein solutions. J. Phys. Chem B, 113: 10081-10087, 2009.

Zhang H, Sachdev D, Wang C, Hubel A, Gaillard-Kelly M, Yee D. “Detection and downregulation of type I IGF receptor expression by antibody-conjugated quantum dots in breast cancer cells”. Breast Cancer Res Treat, 114: 277-285, 2009.

Crabb RAB, Hubel. “Influence of matrix processing on the optical and biomechanical properties of a corneal stromal equivalent,” Tissue Engr, 14(1): 173-182 2008.

Bischof J, Hubel A, Aksan A, “The role of water at multiple length scales”. J. Japanese Soc. Mech. Engr., 111(1076): 598-600, 2008.

Mata C, Longmire EK, McKenna DH, Glass KK, Hubel A, “Experimental study of diffusion based extraction from a cell suspension”. Microfluid Nanofluid, 5(4): 529-540, 2008.

Glass KK, Longmire EK, Hubel, A. “Optimization of a microfluidic device for diffusion based extraction of DMSO from a cell suspension”, Int. J. Heat Mass Transfer, 51: 5749-5757, 2008.

Aksan A, Hubel A. “Biopreservation of cells for use in biotechnology and cell therapy”, Bioprocessing J, 7:43-47, 2008.

Crabb RAB, Hubel. “Influence of matrix processing on the optical and biomechanical properties of a corneal stromal equivalent,” Tissue Engr, 12: 2822-2832 2007.

Fleming KK, Longmire EA, Hubel A. “Characterization of diffusion-based extraction in cell-laden flow through a microfludic device”, J Biomech Engr, 129: 703-711, 2007.

Chang A, Dantzig J, Darr TB, Hubel A. “Modeling the interaction of biological cells with a solidifying interface,” J Computational Phys, 226: 1808-1827, 2007.

Hubel A, Darr TB, Chang A, Dantzig J. “Cell partitioning during the directional solidification of trehalose solutions,” Cryobiology, 55: 182-188, 2007.

Fleming K, Hubel A. “Cryopreservation of hematopoietic stem cells: emerging science, technology and issues,” Trans Med Hemother, 34: 268-275, 2007.

Fleming, KK and Hubel, A, "Cryopreservation of hematopoietic and non-hematopoietic cells", Transf. Apheresis Sci., 34: 309-315, 2006.

Crabb RA, Chau EP, Decoteau DM, Hubel A. Microstructural characteristics of extracellular matrix produced by stromal fibroblasts. Ann Biomed Eng. 34(10):1615-27, 2006.

Crabb RAB, Chau EP, Evans MC, Barocas VH, and Hubel A. "Biomechanical and microstructural characteristics of a collagen film-based corneal stromal equivalent," Tissue Engr, 12(6): 1565-1575, 2006.

Balasubramanian SK, Bischof JC, and Hubel A. “Water transport and IIF parameters for a connective tissue equivalent,” Cryobiology, 52: 62-73, 2006.

Hubel A and Waxberg M. "Preservation of Cell-based Products for Human Therapeutic Application", Regulatory Affairs Focus, 36-37, February 2006.

Hubel A and Darr TB. “Post thaw function and caspase activity of cryopreserved hepatocyte aggregates”, Cell Preserv Tech, 2(3): 164-171, 2004.

Borene M, Barocas V, Hubel A. “Mechanical Characterization and Phenotype Assessment of Corneal Fibroblasts Cultured in a Three-Dimensional Collagen Matrix,” Ann
Biomed Engr, 32(2): 274-283, 2004.

Hubel, A, Carlquist, D., Clay, M, McCullough, J. “Liquid storage, shipment and cryopreservation of cord blood”, Transfusion, 44: 518-525, 2004.

Orwin E, Borene M, Hubel A. “Biomechanical and Optical Characteristics of a Corneal Stromal Equivalent” J Biomech Eng, 125: 439-444, 2003.

Hubel A, Carlquist D, Clay M, McCullough J. “Cryopreservation of Cord Blood After Liquid Storage,” Cytotherapy, 5(5): 370-376, 2003.

Hubel A, Carlquist D, Clay M, and McCullough J. “Short term liquid storage of umbilical cord blood,” Transfusion, 43: 626-632, 2003.

Schmid J, McCullough J, Burger S, and Hubel A. "Non-cryopreserved bone marrow storage in STM-Sav, an infusible-grade cell storage solution,” Cell Preserv Tech, 1(1): 45-52, 2002.

Hubel A. “Cryopreservation of HPCs for clinical use,” Transfusion, 41: 579-580, 2001. [editorial].

Bidault N, Hammer B, and Hubel A. “Water content in an engineered dermal replacement during permeation of Me2SO solutions using MR imaging,” Biotechnol Prog, 17(3): 530-536, 2001.

Conroy M, Darr TB, and Hubel A. “Influence of preculture on the prefreeze and postthaw characteristics of hepatocytes,” Biotechnol Bioeng, 71(3):173-183, 2001.

Darr TB and Hubel A. “Post thaw viability of precultured hepatocytes,” Cryobiology, 42(1): 11-20, 2001.

Koscheyev VS, Leon GR, Hubel A, Nelson ED, and Tranchida D. “Thermoregulation and heat exchange in a nonuniform thermal environment during simulated extended EVA,” Aviat Space Environ Med, 71(6): 579-585, 2000.

Orwin EJ and Hubel A. “In vitro culture characteristics of corneal epithelial, endothelial and keratocytes in a native collagen matrix,” Tissue Eng, 6(4): 307-319, 2000.

Bidault NP, Hammer BE, and Hubel A, “Rapid MR imaging of cryoprotectant permeation in an engineered dermal replacement,” Cryobiology, 40(1): 13-26, 2000.

Hubel A, Darr TB, and Norman J. “Freezing characteristics of genetically modified lymphocytes for the treatment of MPS II,” Cell Transplant, 8: 521-530, 1999.

Burger S, Hubel A, and McCullough J. “Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage,” Cytotherapy, 1: 123-133, 1999.

Hubel A, Norman JA, and Darr TB. “Cryobiophysical characteristics of genetically modified hematopoietic progenitor cells,” Cryobiology, 38: 140-153, 1999.

Korniski B, Darr TB, and Hubel A. “Subzero osmotic characteristics of intact and disaggregated hepatocyte spheroids,” Cryobiology, 38(4): 339-352, 1999.

Stroncek DF, Hubel A, Shankar RA, Burger SR, Pan D, McCullough J, and Whitley CB. “Retroviral transduction and expansion of peripheral blood lymphocytes for the treatment of mucopolysaccharidosis type II, Hunter's syndrome,” Transfusion, 39(4): 343-350, 1999.

Hubel A, Stroncek D, Whitley CB, Pan D, and McCullough J. “Mobilization and transduction of peripheral blood progenitor cells in patients with mucopolysaccharidoses I,” J Hematotherapy, 7(6): 505-514, 1998.

Darr TB and Hubel A. “Freezing characteristics of isolated pig and human hepatocytes,” Cell Transplant, 6(2): 173-183, 1997.

McCarty JP and Hubel A. “Kinetics of endothelial cell detachment under hypothermic conditions,” Cryo-let, 17: 257-264, 1996.

Yarmush M, Toner M, Dunn JCY, Rotem A, Hubel A, and Tompkins RG. “Hepatic tissue engineering: development of critical technologies,” Ann NY Acad Sci, 665: 238-252, 1992.

Hubel A, Toner M, Cravalho EG, Yarmush ML, and Tompkins RG. “Intracellular ice formation during the freezing of hepatocytes cultured in a double collagen gel,” Biotechnol Prog, 7: 554-559, 1991.

Hubel A, Cravalho EG, Nunner B, and Korber C. “Survival of directionally solidified B-lymphoblasts under various crystal growth conditions,” Cryobiology, 29: 183-198, 1992.

Harris CL, Toner M, Hubel A, Cravalho EG, Yarmush ML, and Tompkins RG. “Cryopreservation of isolated hepatocytes: intracellular ice formation under various chemical and physical conditions,” Cryobiology, 28: 436-444, 1991.

Koebe H, Dunn J, Toner M, Sterling L, Hubel A, Cravalho EG, Yarmush M, and Tompkins R. “A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration,” Cryobiology, 27: 576-584, 1990.

Beckmann J, Korber C, Rau G, Hubel A, and Cravalho EG. “Redefining cooling rate in terms of ice front velocity and thermal gradient: first evidence of relevance to freezing injury to lymphocytes,” Cryobiology, 27(3): 279-287, 1990.

Hubel A, Korber C, and Cravalho EG. “Transient electrical potentials measured during the unidirectional freezing of NaCl/H2O solutions,” J Crystal Growth, 87: 69-78, 1988.

Hubel A, Toner M, and Cravalho EG. “Isothermal nucleation stage and its application in the freezing of biological cells,” Trans Am Soc Mech Eng Heat Transfer Division, 87-WA/HTD-90/BED-5: 67-71, 1987.

Korber C, Englich S, Schwindke P, Schiewe M, Rau G, Hubel A, and Cravalho EG. “Low temperature light microscopy and its application to study freezing in aqueous solutions and biological cell suspensions,” J Microscopy, 141: 263-276, 1986.

Refereed Conference Proceedings

Hubel A, Bidault N, Hammer B. “Transport Characteristics of Glycerol and Propylene Glycol in an Engineered Dermal Replacement,” IMECE2002-32557, 2002.

Hubel A and Orwin EJ. “Migration of corneal epithelium on a collagen sponge in vitro and in vivo,” Trans Am Soc Mech Eng, BED-vol 50, 351-352, 2001.

Hubel A, Williams A, and McCullough J. “Role of surface modification on the culture and transduction of hematopoietic cells for gene therapy,” Trans Am Soc Mech Eng, Bioeng Div, 42: 7-8, 1999.

Korniski B and Hubel A. “A model of low-temperature water transport for hepatocyte spheroids,” Ann NY Acad Sci, 858: 183-190, 1998.

Hubel A and McCullough J. “Cryopreservation of cultured blood cells for use in gene therapy and immunotherapy,” Adv Heat and Mass Transfer Biotech, HTD-Vol 355: 97-98, 1997.

Hubel A, Burger S, Whitley CB, and McCullough J. “Expansion and transduction of blood cells for gene therapy,” Trans Am Soc Mech Eng, Bioeng Div, 35: 419-420, 1997.

Hubel A and Darr TB. “Cryobiophysical parameters for freshly isolated human hepatocytes and HEP G2/C3A cells,” Adv Heat and Mass Transfer Biotech, HTD-Vol 322: 73-74, 1995.

Nelson ED and Hubel A. “Post-freeze thaw characteristics of collagen sponge,” Trans Am Soc Mech Eng, Bioeng Div, 29: 99-100, 1995.

Darr TB and Hubel A. “Investigation of subzero water transport properties for isolated hepatocytes and hepatocytes cultured in spheroids,” Trans Am Soc Mech Eng, Bioeng Div, 29: 269-270, 1995.