Blood Flow Measurement

 

Inflammation and Blood Flow Dynamics

In Normal and Transgenic Sickle Cell Mice

 

Work In Collaboration with

 

 Dr.Gregory Vercellotti, Dr. John Belcher

Department of Medicine, University of Minnesota

 

 

Inflammation, Vaso-Occlusion

 and Ischemia/Reperfusion Injury

A Vicious Cycle?

 

 

Techniques Used in the Study

     

  • Dorsal Skin Fold Chambers (DSFC) to visualize the microcirculation in mice
  • Intravital Microscopy to observe changes in blood flow and WBC rolling
  • Optical Doppler Velocimeter (ODV) to measure RBC velocity
  • Digital Image Splitting Device to measure diameter of microvessels

 

 

Mouse with a Dorsal Skin Fold Chamber

Mounted on the Microscope Stage

Collaboration with Ken Diller - U Texas at Austin

 

 

Closeup of Dorsal Skin Fold Chamber

 

Baseline velocity and diameter in arteries in DSFC

Normal Mice compared to other systems in literature

 

 

RBC Velocity in Veins vs. Diameter

in Different Animal Models

 

 

Vascular Imaging in DSFC - Control

 

 

 

Optical Doppler Velocimeter

 

 

Transgenic Murine models for Sickle Cell disease

 

RBC Velocity in Veins vs. Diameter

in Different Animal Models

 Zweifach, B.W et.al, (1977), "Quantitative Studies of Microcirculatory

 Structure and Function", Circulation research, Vol 41(3), 380-390.

 

 

Leukocyte Rolling is Increased in

Transgenic NY-S/SAntilles Mice