University of Minnesota
University of Minnesota: Department of Mechanical Engineering
http://www.me.umn.edu/

 

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Services Offered

The specific benefit to IEM investigators will be to support pilot and long-term studies for grants, papers and student training using rodent and cancer models. This can be a significant savings in time and effort by avoiding the difficulty of setting up separate IACUC protocols. This will further allow faculty to avoid the arduous task of acquiring and propagating tumor cell lines, and establishing protocols for both hindlimb and dorsal skin fold (intravital window) tumor propagation and controlled substance use in their own labs.

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tumor monitoring

 

 

 

 

 

 

 


1. Controlled Substance Management

The core technician/staff will manage all controlled substances, including

a) Purchasing, receiving and storing controlled substances
b) Maintaining the record in the lab
c) Daily entry of controlled substance usage record
d) Appropriate disposal of expired or extra controlled substance waste
e) Preparing for annual inspection with the DEA unit registrant


2. Animal Management

The core technician/staff will manage all animal protocol(s), including

a) Maintaining active animal protocols (IACUC) in the lab
b) Preparing updates and changes in the protocols for specific projects
c) Recording procedures in the protocol
d) Ordering animals under different protocols
e) Preparing for semi-annual inspection with the animal research compliance specialist from IACUC
f ) Maintaining records required by ROHP, EHS etc.


3. Animal Handling

The core technician/staff will handle the animals and induce tumors in the DSFC or hindlimb model. Certain techniques are required for performing the tasks.

a) Microisolator techniques
b) Euthanasia and disposal
c ) Mouse/rat basics (housing, anesthesia etc.)
d) Mouse/rat injection (tail vein, subcutaneous, intraperitoneal, and Intramuscular)
e) Basics of suturing
f ) Aseptic techniques
g) Blood collection
h) Contacting RAR and IACUC for related issue

cancer cell culture

services tumor implant technique

 

 

 

 

 

 

 

4. Cancer Cell Culture

The quality of the seeded cancer is directly dependent on the cells used. As such the technician/staff will grow and maintain the cancer cell line and provide the following services:

a) Sterile cell culture techniques
b) Preparing cell growth medium
c) Cell culture and cell passaging techniques
d) Ordering cell culture material and supplies
e) Cell line preservation techniques
f) Harvesting cancer cells from regular culture
g) Preparing cell suspension for tumor implantation


5. Tumor Implantation Techniques

The technician/staff will perform tumor implantation in hindlimb or DSFC model systems:

a) Assembly and autoclave of Dorsal Skin Fold Chamber (DSFC) instruments
b) Administration of animal anesthesia
c) Surgical implantation of DSFC on rodents
d) Hind Limb tumor implantation on rodents
e) Monitoring the condition of animals after implantation
f) Administration of post-procedure medication for rodents
g) Cleaning and maintaining the DSFC instruments after usage


6. Tumor monitoring

The core technician/staff will assess:

a) Tumor growth delay (Hindlimb tumors)
b) Perfusion and vascular patency (DSFC)
c) Histology
d) Other outcomes may be possible upon consultation.